Journal: Science Advances
Article Title: Machine learning–powered single-molecule cancer diagnosis using DNA origami tags
doi: 10.1126/sciadv.adz8174
Figure Lengend Snippet: ( A ) Top: Schematic showing the conversion of phiX 174 ssDNA into phiX 174 dsDNA via primer extension. Bottom: The corresponding agarose gel electrophoresis analysis [lanes: 1, DNA marker (5000 bp); 2, phiX174 ssDNA; 3, phiX174 dsDNA] and AFM image of phiX 174 dsDNA. ( B ) Schematic representation of the specific recognition sequences of Cas9 nickase and NE 1 on phiX 174 dsDNA. ( C ) Flowchart of the NDAM strategy for multilocus detection on phiX 174 dsDNA based on Cas9 nickase and NE 1. ( D ) AFM imaging of phiX 174 dsDNA with nickase recognition sites colabeled with triangular origamis. ( E ) Confusion matrix representing the performance of YOLOv5l model. ( F ) Precision, recall, and F1 score of YOLOv5l model. Scale bars, 100 nm.
Article Snippet: Cas9 nickase nuclear localization signal was supplied by Beyotime Biotech Inc. (Shanghai, China).
Techniques: Agarose Gel Electrophoresis, Marker, Imaging